January 16 2013

12:00 LSB 2320

Leif Andersson
Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University

Detecting Loci Under Selection Whole Genome Sequencing


Whole genome sequencing (WGS) using pooled or individual samples provides an extremely powerful approach for detecting genetic differences associated with phenotypic traits. The method can be used to reveal signatures of selection in the form of selective sweeps (stretches of homozygosity) or genetic differentiation between populations. Whole genome sequencing is also a very efficient method for the identification of structural changes and we have used WGS to reveal structural changes causing the Rose-comb (PLoS Genetics 8: e1002775) and Fibromelanosis (PLoS Genetics 7:e1002412) phenotypes in chicken and white spotting patterns in pigs (PNAS in press). We have also used the method to scan the genomes of domestic animals for the presence of obvious candidate mutations such as nonsense mutations in single copy genes and have so far found only one, the nonsense mutation in DMRT3 affecting gait control in horses (Nature. 488:642-646). Here I will review our experiences using this approach in a variety of species including chicken (Nature 464: 587-591), pigs (PNAS in press) and the Atlantic herring (PNAS in press).















































































































































































































































































































































































































































































































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