April 18 2019

5:00 pm 1100 TLSB

This seminar is sponsored by Division of Life Sciences

EcoEvoPub Series

Employing the power of metabarcoding and autonomous reef monitoring structures to assess Indonesian marine macro and micro biodiversity


Dita Cahyani
Department of Ecology and Evolutionary Biology, UCLA

The Coral Triangle is the global epicenter of marine biodiversity. However, these reefs are also among the worlds most threatened, imperiling the economic benefits and food security these ecosystems provide. Ensuring the sustainability of these ecosystems requires biodiversity monitoring capable of detecting changes related to environmental change. We used Autonomous Reef Monitoring Structures (ARMS) to assess patterns of marine biodiversity across Indonesia, the largest region of the Coral Triangle. When used in conjunction with DNA metabarcoding, ARMS provide a standardized high-throughput method for elucidating the composition of entire benthic biological communities. Three different barcoding loci (COI, 18S, and 16S) produced almost 90 million DNA sequences, representing more than 250 thousand amplicon sequence variants (ASVs), a molecular equivalent of species, for both macro and microorganism. Microbial community composition did not differ significantly across locations, while metazoan community composition was significantly different among locations across Indonesia. Not only does the combination of ARMS and metabarcoding provide unique insights into patterns of marine biodiversity across a broad range of taxonomic groups in this marine biodiversity hotspot, but it also provides a taxonomically diverse baseline of local biodiversity patterns, providing resource managers a powerful tool for monitoring future change in this biologically and economically important ecosystem.

Thursday, April 18th, 2019 @ 5 PM
1100 Terasaki Life Sciences Building (TLSB)











































































































































































































































































































































































































































































































































































































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